Fig. 3.

osa-miRf10422-akr precursors and its putative target gene. a CYP704A3 is a putative target of osa-miRf10422-akr. CYP704A3 structure and mutation sites are labeled as SNPs (blue) and the changed amino acid residues (red). b Significant differences in grain length between SNP genotypes of the CYP704A3 gene. **indicates a significance at P < 0.01. c qRT-PCR analysis shows the expression of CYP704A3 in maturing seeds. Asominori and IR24 were analyzed in terms of the expression of the CYP704A3 transcript. Seeds with longer lengths have lower levels of relative expression, similar to the other members of subfamily CYP450 such as CYP724B1, of which its mRNA accumulates at higher levels in CYP724B1 mutants with shorter seed compared to that in the wide-type. d Subcellular localization of the CYP704A3Protein. GFP signals of the CYP704A3-GFP fusion proteins localized in the endoplasmic reticulum of rice protoplasts