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. 2016 Apr 19;16:93. doi: 10.1186/s12870-016-0779-3

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© Tang et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — The effects of osa-miRf10422-akrexpression on the accumulation of the CYP704A3 gene. The schematic representation of the reporters and the effectors used in this assay is shown in (a). GFP fluorescence images of the co-expression of osa-miRf10422-akr with the reporter gene EGFP, which was fused with the empty vector control (b) and miRNA target region in the 3′-UTR of the CYP704A3 gene (c). Fluorescence imaging analysis of the agroinfiltrated leaves at 2 dpi under UV illumination. Quantitation of EGFP mRNA as averaged from three leaves from each infiltration treatment (d)