Table 2.
Long-term fermentation of B. subtilis strains in the LB(Y) medium supplemented with 105 g/L glucose under microaerobic condition
| Strain | CDWa (g/L) (at 216 h) | 2,3-BDb (g/L) | ACb (g/L) | Acetateb (g/L) | Lactateb (g/L) | Succinateb (g/L) | Glucose consumed (g/h) (at 216 h) | Glucose uptake rate [g/(L h)] | 2,3-BD productivity [g/(L h)] | 2,3-BD yield (mol/mol) |
|---|---|---|---|---|---|---|---|---|---|---|
| BSF1 | 3.10 ± 0.38 | 40.84 ± 1.25(D) | 3.47 ± 0.49 | 0.63 ± 0.11 | 1.19 ± 0.15 | 0.10 ± 0.02 | 87.3 ± 2.1 | 0.404 | 0.189 | 0.936 |
| BSF9 | 3.82 ± 0.41 | 50.16 ± 0.52(M) | 3.35 ± 0.79 | 0.62 ± 0.11 | 0.04 ± 0.00 | 0.16 ± 0.02 | 104.3 ± 0.5 | 0.483 | 0.232 | 0.962 |
| BSF25 | 4.17 ± 0.22 | 49.59 ± 0.23(M) | 2.66 ± 0.48 | 0.34 ± 0.04 | 1.17 ± 0.17 | 0.11 ± 0.02 | 105.0 ± 0.0 | 0.486 | 0.230 | 0.945 |
Bacteria were cultivated using a mixture of 100-mL LBR medium and 10.5 % glucose in a 250-mL flask kept agitated at a speed of 100 rpm and 37 °C; Data are average values and standard deviations of triplicate experiments. D, d-(−)-2,3-BD; M, meso-2,3-BD
aThe CDW was calculated using the relationship: “CDW = OD600*0.325”
bThese metabolites were measured at 216 h