Table 3.
Strains and plasmids
| Name | Relevant genotype | Source/reference |
|---|---|---|
| Strains | ||
| B. subtilis 168 | Wide-type strain, trpC2 | BGSCa |
| E. coli DH5α | Invitrogen | |
| K. pneumoniae CICC 10011 | CICCb | |
| BSF1 | B. subtilis 168Δupp | (Fu et al. [25]) |
| BSF2 | B. subtilis 168ΔuppΔacoA | This study |
| BSF3 | B. subtilis 168ΔuppΔacoAΔbdhA | (Fu et al. [25]) |
| BSF4 | B. subtilis 168ΔuppΔacoAΔbdhAΔpta | This study |
| BSF5 | B. subtilis 168ΔuppΔacoAΔbdhAΔptaΔldh | This study |
| BSF6 | B. subtilis 168ΔuppΔacoAΔbdhAΔptaΔldh; P 43::alsS-alsD | This study |
| BSF7 | B. subtilis 168ΔuppΔacoAΔbdhAΔptaΔldh; P 43::budC, spc | This study |
| BSF8 | B. subtilis 168ΔuppΔacoAΔbdhAΔptaΔldh; P 43::alsS-alsD; P 43::budC, spc | This study |
| BSF9 | B. subtilis 168ΔuppΔacoAΔbdhAΔptaΔldh; P 43::alsS-alsD; P 43::alsS-alsD-budC-udhA, erm | This study |
| BSF17 | B. subtilis 168ΔuppΔacoAΔbdhA; pHP13-P 43-budC | This study |
| BSF18 | B. subtilis 168ΔuppΔacoAΔbdhA; pHP13-P 43-bdhA | (Fu et al. [25]) |
| BSF25 | B. subtilis 168ΔuppΔacoAΔbdhA; P 43::alsS-alsD-budC-udhA, erm | This study |
| Plasmids | ||
| pUC18 | Ampr | Lab Stock |
| pUC18-uppFB-neo | Kanr | Lab Stock |
| pC194 | Cmr, Bacillus cloning vector | Lab Stock |
| pE194 | Emr, Bacillus cloning vector | Lab Stock |
| pHP13 | Cmr, Emr, E. coli–B. subtilis shuttle vector | Lab Stock |
| pUC18-SP | Ampr, Spcr, containing P 43 promoter | Lab stock |
| pUC18-SP-budC | Ampr, Spcr, containing P 43 promoter and budC | This study |
| pCU | puC18 containing cat-upp cassette | Lab stock |
| pCU-ptaFB | pCU containing pta flanks | This study |
| pCU-ldhFB | pCU containing ldh flanks | This study |
| pCU-bdhAFB-PSD | pCU-bdhAFB containing alsS-alsD under P 43 | This study |
| pUC18-SP-budC | Ampr, Spcr, containing budC under P 43 promoter | This study |
| pUC18-P 43 | Ampr, Emr, containing P 43 promoter | This study |
| pUC18-P 43-SD | Ampr, Emr, containing alsS and alsD under P 43 promoter | This study |
| pUC18-P 43-SDU | Ampr, Emr, containing alsS, alsD and udhA under P 43 promoter | This study |
| pUC18-P 43-SDBU | Ampr, Emr, containing alsS, alsD, budC, and udhA under P 43 promoter | This study |
| pPSDBUE | pUC18-P 43-SDBU containing erythromycin resistance gene | This study |
| pHP13-P 43-budC | Emr, Cmr, containing budC under P 43 promoter | This study |
a Bacillus Genetic Stock Center
bChina Center of Industrial Culture Collection