Table 3.
Summary of sequence assembly after Illumina sequencing
Sample name | Raw reads | Clean reads | Clean bases | Error rate (%) | Q20 (%) | Q30 (%) | GC content (%) |
---|---|---|---|---|---|---|---|
R118_0 | 10144830 | 9985483 | 0.5G | 0.01 | 97.94 | 94.16 | 43.74 |
Yumian 1_0 | 11213370 | 11081107 | 0.55G | 0.01 | 98.01 | 94.35 | 43.77 |
R118_5 | 14141978 | 14104947 | 0.71G | 0.01 | 99.03 | 96.58 | 44.01 |
Yumian 1_5 | 14027353 | 13969205 | 0.7G | 0.01 | 98.99 | 96.49 | 43.94 |
Raw reads: Statistical data for the original sequences. Clean reads: Calculation method is the same as for raw reads, but the statistics file is the filtered data. Clean bases:sequence number*sequence length (transformed into G bases). Q20、Q30:Percentage of the number of bases of Phred score greater than 20, 30 respectively. GC content:Percentage of G and C bases among the total base number