Table 2.
Summary of the results obtained using particular diagnostic tools
| Case No | Microscopy | DNA analysis | Serology | |||
|---|---|---|---|---|---|---|
| Gene | Sequence lenght (bp) | Database accesion No | Identity (Database accesion No) | |||
| 1 | Dirofilaria spp. (Susp. D.repens) | cox 1 | 682 | [GenBank: KR998257] | 99 % D. repens [GenBank: KF692102] | Positive |
| 2 | Dirofilaria spp. (Susp. D.repens) | cox1 | 482 | [GenBank: KR998258] | 99 % D. repens [GenBank: KF692102] | Borderline |
| 5S (153 bp) | 153 | [GenBank: KR998254] | 97 % D. repens [GenBank: KC429769] | |||
| 5S (247 bp) | 247 | [GenBank: KR998255] | 99 % D. repens [GenBank: AJ242966] | |||
| 3 | Dirofilaria spp. (Susp. D.repens) | - | - | - | - | Borderline |
| 4 | Dirofilaria spp. (Susp. D.repens) | cox 1 | 664 | [GenBank: KR998259] | 99 % D. repens [GenBank: KF692102] | Negative |
| 5S (153 bp) | 153 | [GenBank: KR998256] | 99 % D. repens [GenBank: AJ242967] | |||
| 5 | Dirofilaria spp. (Susp. D.repens) | - | - | - | - | Borderline |