Figure 1.

Loss of individual Akt isoforms in vivo does not affect PNS myelination. A, Western blot analysis of the expression of the three Akt isoforms in DRG neurons (Neu), Schwann cells (SC), and nonmyelinated (C) or myelinated (C+C) cocultures. Akt1 and Akt3 are preferentially expressed by neurons, whereas Akt2 is primarily expressed by Schwann cells. n = 3. Data are mean ± SEM. B, Semithin sections of sciatic nerves from 2-month-old mice deficient in each Akt isoform revealed no obvious defects of myelination. Left panels, Magnification of the inset from the semithin section. Right panels (a–f), Representative images of Remak bundles. No abnormalities of myelination or ensheathment were evident at the time point analyzed, although no quantifications were performed. Scale bars: semithin, 100 μm; EM, 2 μm.