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. 2016 Apr 20;36(16):4647–4657. doi: 10.1523/JNEUROSCI.3774-15.2016

Figure 1.

Figure 1.

DopEcR mutants and the endogenous DopEcR expression pattern in the brain deduced using a GAL4 knock-in allele, DopEcRGAL4. A, DopEcR genomic locus illustrating the piggyBac transposon insertion mutant DopEcRc02142 (PB1) and GAL4 knock-in mutant DopEcRGAL4 (GAL4). An arrow, gray boxes, and black boxes depict the transcription start site, exons, and coding region, respectively, of the DopEcR gene. B, Anterior view of the adult brain displaying GFP reporter expression driven by GAL4 activity associated with DopEcRGAL4. C, D, Double labeling of the adult brain (DopEcRGAL4/UAS-myrGFP-2A-dsRednls) for nuclear RFP reporter for DopEcR (magenta) and neuronal marker Elav (C, green) or glial marker Repo (D, green). Colabeling (white) is visualized using ImageJ plug-in Colocalization.