Cytochrome P450 derived AA metabolites are elevated in impaired healing. Lipoxygenase mediated conversion of AA-derived results in significant elevation of HETEs shortly after wounding of the LIGHT−/− mice. LIGHT−/− tissues collected at multiple time points after wounding were assayed for 15- HETE (A) which showed two peaks, one at day 1 and the other at day 7 postwounding and 11-HETE (B) which was overall elevated throughout the period of healing with respect to control C57BL/6 mice. 12-HETE (C) and 5,6 EET (D) were significantly elevated at days 1 and 3 postwounding. No other significant differences were noted at other time points. 11,12 EET (E) and 14,15 EET (F) were both significantly elevated at days 2 and 3 postwounding in the LIGHT−/− wounds. n = 4. All data are Mean ± SD. *p<0.05, **p<0.01, ***p<0.001.