Fig. 3.

Inhibitory effect of fresh seed extract (FSE) and wine seed extract (WSE) against intracellular reactive oxygen species generation in HepG2 cells as measured by DCFH-DA assay. HepG2 cells were labeled with DCFH-DA and treated with different concentrations of FSE and WSE (a) and treated with same concentration (100 μg/mL) of FSE and WSE (b). Fluorescence intensities of DCF due to oxidation of DCFH by cellular ROS generated by H₂O₂ were detected after 2 h (Ex = 485 nm and Em = 535 nm). Different letters above bars indicates significant different from control