Table 2.
Compare of common separation methods for Cell-derived MPs
| Method | Isolation MP (speed, time) | Generic MP detection | Platelet MP detection | Endothelial MP detection | Leukocyte MP detection |
|---|---|---|---|---|---|
| Flow cytometry | 18,000×g, 30 minutes | Annexin V | CD62P, CD61, CD63 | CD31, CD62E or CD144 | CD4, CD8, etc. |
| Flow cytometry | − | Annexin V | – | CD51, CD144 or CD146 | CD45 |
| Capture based assay | − | Annexin V, tissue factor | CD62P or GPIba | CD31 or CD62E | CD45 |
| Flow cytometry | − | − | CD41 or CD42b and CD31 | CD31 +/ CD42 − or CD62E | CD45 |
| ELISA | − | − | GP IX (capture) CD62P, CD40 L | − | − |
| Flow cytometry | 100,000×g, 30 minutes | Annexin V | CD41a | CD144 | CD14 (monocyte) |
MPs: Microparticles; ELISA: Enzyme linked immunosorbent assay.