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. 2015 Feb 5;128(3):353–360. doi: 10.4103/0366-6999.150103

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Copyright: © 2015 Chinese Medical Journal

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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The relative amounts of protein expression levels of molecular markers associated with apoptosis, such as caspase-3, Bcl-2 and Bax. (a) Results of Western blotting of caspase-3, Bcl-2, Bax and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (b) The statistical analysis graph of relative expression levels of these markers normalized to that of GAPDH. Hypoxia for 8 hours and reoxygenation for 16 hours (hypoxia/reoxygenation [H/R]) up-regulated the expressions of caspase-3 and Bax, and down-regulated Bcl-2 expression. calreticulin overexpression induced by plasmid transfection 24 h before H/R further aggravated the changes induced by H/R, while siRNA interference and taurine administration had the opposite effects. Each experiment was repeated in triplicate (*P < 0.05 vs. control. ^†P <0.05 vs. H/R).