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. Author manuscript; available in PMC: 2016 Nov 16.
Published in final edited form as: Chem Res Toxicol. 2015 Oct 22;28(11):2104–2111. doi: 10.1021/acs.chemrestox.5b00270

Figure 7.

Figure 7

Cell oxidant generation and release of IL-6 and IL-8 after exposure to humic acid. Oxidant generation by BEAS-2B cells was increased following exposure to 100 μg/mL of humic acid (A). Incubation of cells with 200 μM FAC diminished the oxidant generation following exposure to PBS and humic acid. Pretreatment of cells with rotenone for 15 min inhibited oxidant generation after humic acid, suggesting a mitochondrial origin (B). Exposure of BEAS-2B cells to 100 μg/mL of humic acid for 24 h increased the release of IL-6 (C) and IL-8 (D), whereas the 200 μM FAC treatment diminished the pro-inflammatory response. In (C) and (D), * indicates a significant increase relative to all other exposures, and ** indicates a significant increase relative to media exposure.