Figure 2. Reactivity of a patient’s serum with live HEK293 cells expressing GABAAR.
Reactivity of live HEK293 cells expressing human a1/b3 subunits of the GABAAR with a patient’s serum (A) and a monoclonal antibody against the a1 subunit (B). Merged reactivities (C). A similar assay with serum from a control individual is shown in (D–F). The nuclei of the cells are shown with DAPI in C and F. Note the specific reactivity of patient’s antibodies with cells expressing GABAAR and the co-localisation with the reactivity of the commercial antibody.