. Author manuscript; available in PMC: 2016 Apr 20.

Published in final edited form as: Nature. 2015 Apr 22;520(7549):692–696. doi: 10.1038/nature14426

Extended Data Table 1.

Immunogenic B16F10 mutations

Mutation	Gene	Mutated sequence used for vaccination	Substitution (WT, AA#, Mut)	Reactive T cell subtype	MHC I score (best prediction)	Response after vaccination with
Mutation	Gene	Mutated sequence used for vaccination	Substitution (WT, AA#, Mut)	Reactive T cell subtype	MHC I score (best prediction)	Peptide	RNA
B16-M05	Eef2	FVVKAYLPVNESFAFTADLRSNTGGQA	G795A	CD4⁺	1.1	x
B16-M08	Ddx23	ANFESGKHKYRQTAMFTATMPPAVERL	V602A	CD4⁺	1.3		x
B16-M12	Gnas	TPPPEEAMPFEFNGPAQGDHSQPPLQV	S111G	CD4⁺	1.2	x
B16-M17	Tnpo3	VVDRNPQFLDPVLAYLMKGLCEKPLAS	G504A	CD4⁺	1.0	x
B16-M20	Tubb3	FRRKAFLHWYTGEAMDEMEFTEAESNM	G402A	CD4⁺	1.9	x
B16-M21	Atp11a	SSPDEVALVEGVQSLGFTYLRLKDNYM	R552S	CD4⁺	0.1		x
B16-M22	Asf1b	PKPDFSQLQRNILPSNPRVTRFHINWD	A141P	CD4⁺	1.7	x
B16-M24	Dag1	TAVITPPTTTTKKARVSTPKPATPSTD	P425A	CD4⁺	2.2	x
B16-M25	Plod1	STANYNTSHLNNDVWQIFENPVDWKEK	F530V	CD4⁺	0.1	x	x
B16-M27	Obsl1	REGVELCPGNKYEMRRHGTTHSLVIHD	T1764M	CD8⁺	2.3	x	x
B16-M28	Ppp1r7	NIEGIDKLTQLKKPFLVNNKINKIENI	L170P	CD4⁺	3.2	x	x
B16-M29	Mthfd1l	IPSGTTILNCFHDVLSGKLSGGSPGVP	F294V	CD4⁺	1.7	x
B16-M30	Kif18b	PSKPSFQEFVDWENVSPELNSTDQPFL	K739N	CD4⁺	1.2	x	x
B16-M33	Pbk	DSGSPFPAAVILRDALHMARGLKYLHQ	V145D	CD8⁺	0.1		x
B16-M36	Tm9sf3	CGTAFFINFIAIYHHASRAIPFGTMVA	Y382H	CD4⁺	0.2	x
B16-M44	Cpsf3l	EFKHIKAFDRTFANNPGPMVVFATPGM	D314N	CD4⁺	0.5	x	x
B16-M45	Mkrn1	ECRITSNFVIPSEYWVEEKEEKQKLIQ	N346Y	CD4⁺	1.4	x
B16-M46	Actn4	NHSGLVTFQAFIDVMSRETTDTDTADQ	F835V	CD4⁺	0.2	x	x
B16-M47	Rpl13a	GRGHLLGRLAAIVGKQVLLGRKVVVVR	A24G	CD4⁺	0.5		x
B16-M48	Def8	SHCHWNDLAVIPAGVVHNWDFEPRKVS	R255G	CD4⁺	3.8	x	x
B16-M50	Sema3b	GFSQPLRRLVLHVVSAAQAERLARAEE	L663V	CD4⁺	2.9	x	x

B16F10 mutations determined to be immunogenic upon peptide or RNA immunization (see Fig. 1 and Extended Data Fig. 1). WT, wild type; AA#, position of mutated amino acid; Mut, mutation.