Extended Data Table 2.
Immunogenic CT26 mutations
| Mutation | Gene | Mutated sequence used for vaccination | Substitution (WT, AA#, Mut) | Reactive T cell subtype | MHC I score (best prediction) |
|---|---|---|---|---|---|
| CT26-M03 | Slc20a1 | DKPLRRNNSYTSYIMAICGMPLDSFRA | T425I | CD4+ | 0,3 |
| CT26-M12 | Gpc1 | YRGANLHLEETLAGFWARLLERLFKQL | E165G | CD8+ | 1,9 |
| CT26-M13 | Nphp3 | AGTQCEYWASRALDSEHSIGSMIQLPQ | G234D | CD4+ | 0,1 |
| CT26-M19 | Tmem87a | QAIVRGCSMPGPWRSGRLLVSRRWSVE | G63R | CD8+ | 0,7 |
| CT26-M20 | Slc4a3 | PLLPFYPPDEALEIGLELNSSALPPTE | T373I | CD4+ | 0,9 |
| CT26-M24 | Cxcr7 | MKAFIFKYSAKTGFTKLIDASRVSETE | L340F | CD4+ | 1,8 |
| CT26-M26 | E2f8 | VILPQAPSGPSYATYLQPAQAQMLTPP | I522T | CD8+ | 0,1 |
| CT26-M27 | Agxt2l2 | EHIHRAGGLFVADAIQVGFGRIGKHFW | E247A | CD4+ | 0,2 |
| CT26-M35 | Nap1l4 | HTPSSYIETLPKAIKRRINALKQLQVR | V63I | CD4+ | 0,7 |
| CT26-M37 | Dhx35 | EVIQTSKYYMRDVIAIESAWLLELAPH | T646I | CD4+ | 0,1 |
| CT26-M39 | Als2 | GYISRVTAGKDSYIALVDKNIMGYIAS | L675I | CD8+ | 0,2 |
| CT26-M42 | Deptor | SHDSRKSTSFMSVNPSKEIKIVSAVRR | S253N | CD4+ | 0,3 |
| CT26-M43 | Tdg | AAYKGHHYPGPGNYFWKCLFMSGLSEV | H169Y | CD4+ | 0,3 |
| CT26-M55 | Dkk2 | EGDPCLRSSDCIDEFCCARHFWTKICK | G192E | CD4+ | 9,7 |
| CT26-M58 | Rpap2 | CGYPLCQKKLGVISKQKYRISTKTNKV | P113S | CD4+ | 11,3 |
| CT26-M68 | Steap2 | VTSIPSVSNALNWKEFSFIQSTLGYVA | R388K | CD4+ | 6,8 |
| CT26-M75 | Usp26 | KTTLSHTQDSSQSLQSSSDSSKSSRCS | S715L | n.d. | 5,8 |
| CT26-M78 | Nbea | PAPRAVLTGHDHEIVCVSVCAELGLVI | V576I | CD4+ | 6,3 |
| CT26-M90 | Aldh18a1 | LHSGQNHLKEMAISVLEARACAAAGQS | P154S | CD4+ | 8,3 |
| CT26-M91 | Zc3h14 | NCKYDTKCTKADCLFTHMSRRASILTP | P497L | CD4+ | 8,8 |
| CT26-M93 | Drosha | LRSSLVNNRTQAKIAEELGMQEYAITN | V1189I | CD4+ | 9,9 |
CT26 mutations determined to be immunogenic upon RNA immunization (see Fig. 1). WT, wild type; AA#, position of mutated amino acid; Mut, mutation.