Extended Data Table 3.
Immunogenic 4T1 mutations
| Mutation | Gene | Mutated sequence used for vaccination | Substitution (WT, AA#, Mut) | Reactive T cell subtype |
| 4T1-M2 | Gen1 | IPHNPRVAVKTTNNLVMKNSVCLERDS | K707N | CD4 |
| 4T1-M3 | Polr2a | LAAQSLGEPATQITLNTFHYAGVSAKN | M1102I | CD4 |
| 4T1-M8 | Tmtc2 | QGVTVLAVSAVYDIFVFHRLKMKQILP | V201I | CD8 |
| 4T1-M14 | Zfr | AHIRGAKHQKVVTLHTKLGKPIPSTEP | K411T | CD4 |
| 4T1-M16 | Cep120 | ELAWEIDRKVLHQNRLQRTPIKLQCFA | H68N | CD4 |
| 4T1-M17 | Malt1 | FLKDRLLEDKKIAVLLDEVAEDMGKCH | T534A | CD4 |
| 4T1-M20 | Wdr11 | NDEPDLDPVQELIYDLRSQCDAIRVTK | T340I | CD8 |
| 4T1-M22 | Kbtbd2 | DAAALQMIIAYAYRGNLAVNDSTVEQL | T91R | CD4 |
| 4T1-M25 | Adamts9 | KDYTAAGFSSFQKLRLDLTSMQIITTD | I623L | CD4 |
| 4T1-M26 | Pzp | AVKEEDSLHWQRPEDVQKVKALSFYQP | G1199E | CD8 |
| 4T1-M27 | Gprc5a | FAICFSCLLAHALNLIKLVRGRKPLSW | F119L | CD8 |
| 4T1-M30 | Enho | MGAAISQGAIIAIVCNGLVGFLL | L10I | CD4 |
| 4T1-M31 | Dmrta2 | EKYPRTPKCARCGNHGVVSALKGHKRY | R73G | CD4 |
| 4T1-M32 | Rragd | SHRSCSHQTSAPSPKALAHNGTPRNAI | L268P | CD4 |
| 4T1-M35 | Zzz3 | KELLQFKKLKKQNLQQMQAESGFVQHV | K311N | CD8 |
| 4T1-M39 | Ilkap | RKGEREEMQDAHVSLNDITQECNPPSS | 127S | CD4 |
| 4T1-M40 | Cenpf | RVEKLQLESELNESRTECITATSQMTA | D1327E | CD4 |
4T1 mutations determined to be immunogenic upon RNA immunization (see Fig. 1). WT, wild type; AA#, position of mutated amino acid; Mut, mutation.