Extended Data Table 4.
Pentatope | Mutation | Gene | Mutated sequence used for vaccination | Substitution (WT, AA#, Mut) | Reactive T cell subtype |
1 | CT26-M19 | Tmem87a | QAIVRGCSMPGPWRSGRLLVSRRWSVE | G63R | CD8+ |
1 | CT26-M39 | Als2 | GYISRVTAGKDSYIALVDKNIMGYIAS | L675I | CD8+ |
1 | CT26-M13 | Nphp3 | AGTQCEYWASRALDSEHSIGSMIQLPQ | G234D | CD4+ |
1 | CT26-M55 | Dkk2 | EGDPCLRSSDCIDEFCCARHFWTKICK | G192E | CD4+ |
1 | CT26-M68 | Steap2 | VTSIPSVSNALNWKEFSFIQSTLGYVA | R388K | CD4+ |
2 | CT26-M20 | Slc4a3 | PLLPFYPPDEALEIGLELNSSALPPTE | T373I | CD4+ |
2 | CT26-M26 | E2f8 | VILPQAPSGPSYATYLQPAQAQMLTPP | I522T | CD8+ |
2 | CT26-M03 | Slc20a1 | DKPLRRNNSYTSYIMAICGMPLDSFRA | T425I | CD4+ |
2 | CT26-M37 | Dhx35 | EVIQTSKYYMRDVIAIESAWLLELAPH | T646I | CD4+ |
2 | CT26-M27 | Agxt2l2 | EHIHRAGGLFVADAIQVGFGRIGKHFW | E247A | CD4+ |
Ten immunogenic mutated epitopes were used for generation of two pentatopes used for therapeutic vaccination shown in Fig. 3b. WT, wild type; AA#, position of mutated amino acid; Mut, mutation.