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. 2016 Mar 17;13(5):3867–3873. doi: 10.3892/mmr.2016.4997

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Copyright: © Bian et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Rac1 promoter is methylated by SAM in LX-2 cells. Normal lymphocytes collected from healthy human blood were used as the negative control, whereas normal lymphocytes treated with bisulfite-converted genomic DNA were used as the positive control. Rac1 promoter was significantly methylated in the 4 mM SAM-treated group compared with in the untreated group. The polymerase chain reaction product of the Rac1 promoter was 164 bp. M, methylated; U, unmethylated; ctr, control; Rac1, ras-related C3 botulinum toxin substrate 1; SAM, S-adenosylmethionine.