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Characterization of MSCs. (A) Undifferentiated passage three MSCs are adherent and spindle-like. (B) Osteogenesis was detected by alizarin red. (C) Adipogenesis was detected by oil red O. (Scale bars in A–C, 100 µm). (D) Flow cytometry indicated that MSCs uniformly expressed CD29, CD44 and CD90, and negatively expressed CD34 and CD45 surface markers. MSCs, mesenchymal stem cells; SSC, side scatter; FSC, forward scatter; PE, phycoerythrin; APC, allophycocyanin.
