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. 2016 Mar 18;13(5):3755–3762. doi: 10.3892/mmr.2016.5004

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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KGF secretion and knockdown efficiency of KGF siRNA. (A) A co-culture system was developed with AT-II cells in the upper compartment, and MSCs in the bottom compartment of a Transwell chamber. (B) KGF secretion was detected by ELISA under different conditions. Experiments were run three times and data were expressed as the mean ± standard deviation (n=3/group, ^**P<0.01 vs. MSCs; ^##P<0.01 vs. MSCs (cytokines); ^††P<0.01 vs. MSCs (cytokines). KGF knockdown efficiency was evaluated by (C) ELISA and (D) semiquantitative reverse transcription-polymerase chain reaction (n=3 per group). The mRNA level was normalized to β-actin expression. Data were expressed as the mean ± standard deviation. (^**P<0.01 vs. all other groups). KGF, keratinocyte growth factor; siRNA, small interfering RNA; AT-II cell, alveolar type II epithelial cell; MSCs, mesenchymal stem cells; ELISA, enzyme-linked immunosorbent assay; Lipof, lipofectamine.