Figure 3.

Curcumin induced expression and localization of forkhead box O1 (FoxO1). (A) U87 cells were left untreated or were treated with 20 or 40 µM curcumin for 24 h. Nuclear and cytoplasmic proteins were extracted according to the nuclear extraction kit protocol. Subsequently, FoxO1 and phosphorylated (p)-FoxO1 expression in the nucleus and cytoplasm were detected by western blotting. (B) After the U87 cells were treated with 20 or 40 µM curcumin, immunofluorescence was conducted to determine the effects of curcumin on nuclear translocation of FoxO1 (magnification, ×20). GAPDH, glyceraldehyde 3-phosphate dehydrogenase; DAPI, 4′,6-diamidino-2-phenylindole.