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. 2016 Mar 21;13(5):3763–3770. doi: 10.3892/mmr.2016.5037

Figure 4.

Figure 4

Effects of forkhead box O1 (FoxO1) on the expression of target proteins associated with cell cycle progression and apoptosis. (A) U87 cells were treated with 40 µM curcumin and were transfected with FoxO1 small interfering (si)RNA for 24 h. Western blotting was conducted to determine the effect of FoxO1 knockdown on cyclin-dependent kinase 1 (CDK1) and cyclin G2 expression. (B) Expression levels of cleaved caspase-3 and Fas ligand (FasL) were determined by western blotting following FoxO1 knockdown and curcumin treatment. (C) U87 cells were transfected with CDK1 siRNA under normal conditions for 24 h. Western blotting was conducted to determine the effects of CDK1 knockdown on phosphorylated (p)-FoxO1, FoxO1, cyclin G2, cleaved caspase-3 and FasL expression. (D) Nuclear expression of FoxO1 was detected by western blotting following CDK1 knockdown. GAPDH, glyceraldehyde 3-phosphate dehydrogenase.