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. 2016 Mar 23;13(5):4159–4166. doi: 10.3892/mmr.2016.5041

Figure 1.

Figure 1

Effects of tCA and TSA on HDAC activity and the expression of related proteins. Hela nuclear extract was treated with various doses of (A) tCA and (B) TSA for 1 h and HDACI/II activity was detected. (C) tCA and (D) TSA inhibited HDAC activity in cultured cells. HT29 cells were treated with various doses of tCA for either 6 or 12 h and HDACI/II activity was detected. (E) Effect of tCA on histone H3 and H4 acetylation in HT29 cells as determined by western blot analysis. Quantification of (F) Ac-H3 and (G) Ac-H4 expression. (H) Effect of tCA on histone H3 and H4 acetylation in MIA PaCa-2 cells as determined by western blot analysis. Quantification of (I) Ac-H3 and (J) Ac-H4 expression. The HDAC inhibitor TSA was used as a positive control. *P<0.05 vs. control. tCA, trans-cinnamic acid; HDAC, histone deacetylases; AC, acetyl; TSA, trichostatin A.