Extended Data Table 1.
Genes that are significantly (>8-fold and P<0.015) more highly expressed by Scf-GFP+ stromal cells in spleen as compared to bone marrow.
| Gene | Gene name | Unigene | Spleen Scf-GFP+ | BM Scf-GFP+ | Fold change |
|---|---|---|---|---|---|
| Coch | Coagulation factor C homolog | Mm.21325 | 12.1±0.3 | 6.6±0.0 | 45.4 |
| Ccl21a | Chemokine (C-C motif) ligand 21A | Mm.458815 | 12.5±0.1 | 7.1±0.4 | 41.1 |
| Acta2 | Actin, alpha 2, smooth muscle, aorta | Mm.213025 | 11.9±0.3 | 6.7±0.1 | 35.2 |
| Cxcl13 | Chemokine (C-X-C motif) ligand 13 | Mm.10116 | 11.8±0.3 | 6.8±0.2 | 30.3 |
| Tcf21 | Transcription factor 21 | Mm.16497 | 11.3±0.6 | 6.6±0.0 | 25.9 |
| Clca1 | Chloride channel calcium activated 1 | Mm.454553 | 11.1±0.3 | 6.6±0.0 | 22.5 |
| Ifi27l2a | Interferon, alpha-inducible protein 27 like 2A | Mm.271275 | 11.3±0.2 | 7.2±0.4 | 16.6 |
| Pln | Phospholamban | Mm.34145 | 10.7±0.1 | 6.6±0.0 | 16.3 |
| Parm1 | Prostate androgen-regulated mucin-like 1 | Mm.5002 | 10.8±0.3 | 6.8±0.1 | 16 |
| Fn1 | Fibronectin 1 | Mm.193099 | 10.7±0.4 | 6.8±0.2 | 14.9 |
| Col14a1 | Collagen, type XIV, alpha 1 | Mm.297859 | 10.4±0.2 | 6.7±0.1 | 12.6 |
| Nr4a1 | Nuclear receptor subfamily 4, group A, 1 | Mm.119 | 10.5±0.6 | 7.0±0.3 | 11.2 |
| Agtr1a | Angiotensin II receptor, type 1a | Mm.35062 | 10.7±0.7 | 7.3±0.6 | 11 |
| Fos | FBJ osteosarcoma oncogene | Mm.246513 | 11.4±0.4 | 8.0±0.4 | 10.7 |
| Atp1b2 | ATPase, Na+/K+ transporter, beta 2 | Mm.235204 | 10.6±0.2 | 7.2±0.2 | 10.6 |
| Tnxb | Tenascin XB | Mm.290527 | 9.9±0.5 | 6.6±0.0 | 9.5 |
| Myh11 | Myosin, heavy polypeptide 11, smooth muscle | Mm.250705 | 10.7±0.7 | 7.5±0.2 | 9.4 |
| Hspb1 | Heat shock protein 1 | Mm. 13849 | 10.8±0.7 | 7.6±0.2 | 9.3 |
| Clca2 | Chloride channel calcium activated 2 | Mm.20897 | 9.8±0.4 | 6.6±0.0 | 8.8 |
| Tagln | Transgelin | Mm.283283 | 10.4±0.5 | 7.3±0.9 | 8.6 |
| Nr2f2 | Nuclear receptor subfamily 2, group F, 2 | Mm.158143 | 10.7±0.3 | 7.6±0.3 | 8.5 |
| Mustn1 | Musculoskeletal, embryonic nuclear protein 1 | Mm.220895 | 10.8±0.5 | 7.7±0.7 | 8.2 |
| Aspn | Asporin | Mm.383216 | 9.7±0.6 | 6.6±0.0 | 8.2 |
| Sparcl1 | SPARC-like 1 | Mm.29027 | 12.1±0.1 | 9.1±0.4 | 8.1 |
Data show mean±s.d. for log2 transformed expression values (n=3 independent samples/cell population). Maximal background expression was considered to be 6.6 (log2(100)); all expression values below this threshold were set to 6.6 for purposes of calculating fold-change. Two-tailed Student's t-tests were used to assess statistical significance. Data for bone marrow Scf-GFP+ stromal cells are from19.