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. 2016 Apr 20;11(4):e0153992. doi: 10.1371/journal.pone.0153992

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© 2016 Yuan et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 7 — (A) Two-hybrid assay of the interaction between Kin2-C1 and Rho3. The assay was performed as described in Materials and Methods. pGBDU-C1 (DBD, vector), pGBDU-RHO3^{Q74L, ΔC}, and pGBDU-RHO3^{T30N, ΔC} were used to pair with pOAD (AD, vector) and pOAD-KIN2-C1. Cells were grown on SC-Leu-Ura-Ade plate at 30°C for 3 days. (B) BiFC assay between Kin2-C9 and Rho3. Cells of strain JGY3088 (kin1Δ kin2Δ) carrying pVN1-KIN2-C9/pVC1, pVN1-KIN2-C9/pVC1-PMT-RHO3^Q74L, and pVN1/ pVC1-PMT-RHO3^Q74L pairs were grown on SC-His-Ura plate at 30°C for 16 hr. Green fluorescence was examined. Bar, 5 μm. (C) Two-hybrid assay of the interaction between Kin2 segments and Bmh1. pGBDU-C1 (DBD, vector) and pGBDU-KIN2 segments were used to pair with pOAD (AD, vector) and pOAD-BMH1^5-267. Cells were grown on SC-Leu-Ura-His containing 3 mM 3-AT (-His+3-AT) and SC-Leu-Ura-Ade (-Ade) plates at 30°C for 3 days.