Fig 4. Effect of polymerase mutations on progeny vRNA production in primary human airway cells.

(A−D) SAE cells were infected with the indicated viruses at an MOI of 0.03 and incubated at 37°C (A and B) or 33°C (C and D). (E−F) CEFs were infected with the indicated viruses at an MOI of 0.005 and incubated at 37°C. The culture supernatants were harvested at the indicated times and assayed by quantitative real-time RT-PCR to determine the amount of progeny vRNA. Each data point is the mean ± SD of the log10 number of vRNA copies/ml from three separate experiments.