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. 2016 Apr 20;11(4):e0153086. doi: 10.1371/journal.pone.0153086

Table 4. Compared the embryo development rate from growth factors supplement in mouse embryos treated with LIF SiRNA.

Stage Untreated (n = 62) Scramblea (n = 60) LIF-siRNA (1.5 fmol)
Vehicle (n = 75) EGF (50 ng/ml) (n = 66) GM-CSF (50 ng/ml) (n = 72) IGF II (50 ng/ml) (n = 75) TGFβ (50 ng/ml) (n = 68) LIF (50 ng/ml) (n = 77)
Two-cell 93.5 93.5 93.0 92.4 83.3 85.7 83.9 93.0
Four-cell 90.3 90.3 83.1 86.4 73.6 75.7 74.2 77.5
Morula 87.1 87.1 69.0 84.8 62.5 54.3 66.1 73.2
Blastocyst 82.3 b 81.7 b 43.6a 75.8b 58.3a 42.9a 48.4a 67.6 b

aP<0.05 compared to scramble group

bP<0.05 compared to only LIF-siRNA treated group