Table 4. Compared the embryo development rate from growth factors supplement in mouse embryos treated with LIF SiRNA.
Stage | Untreated (n = 62) | Scramblea (n = 60) | LIF-siRNA (1.5 fmol) | |||||
---|---|---|---|---|---|---|---|---|
Vehicle (n = 75) | EGF (50 ng/ml) (n = 66) | GM-CSF (50 ng/ml) (n = 72) | IGF II (50 ng/ml) (n = 75) | TGFβ (50 ng/ml) (n = 68) | LIF (50 ng/ml) (n = 77) | |||
Two-cell | 93.5 | 93.5 | 93.0 | 92.4 | 83.3 | 85.7 | 83.9 | 93.0 |
Four-cell | 90.3 | 90.3 | 83.1 | 86.4 | 73.6 | 75.7 | 74.2 | 77.5 |
Morula | 87.1 | 87.1 | 69.0 | 84.8 | 62.5 | 54.3 | 66.1 | 73.2 |
Blastocyst | 82.3 b | 81.7 b | 43.6a | 75.8b | 58.3a | 42.9a | 48.4a | 67.6 b |
aP<0.05 compared to scramble group
bP<0.05 compared to only LIF-siRNA treated group