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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Mar 16;1860(6):1334–1342. doi: 10.1016/j.bbagen.2016.03.021

Figure 6. Ergothioneine transport by wild-type and chimeric OCTN transporters.

Figure 6

A Ergothioneine (1 μM) transport was measured for 60 minutes at 37°C in CHO cells stably transfected with the indicated transporter. Substitutions R341A, L409W, and T429I introduced back OCTN1 residues in the portion of OCTN2 cDNA of chimeric transporters CHIM-2 and CHIM-3. Saturable ergothioneine transport was calculated by subtracting nonspecific uptake, measured in the presence of 1 mM unlabeled substrate, from all points. Values are means ± SD of 9-12 independent determinations (SD not shown when smaller than the size of the symbols). *p<0.01 versus CHO; §p<0.01 versus OCTN1 using Student's t-test (only cells whose transport activity was significantly higher that CHO cells were compared to OCTN1). B. Values of ergothioneine transport in panel A were corrected for the baseline transport value measured in untransfected CHO cells and normalized by the amount of protein relative to OCTN1 (Fig. 3).