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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Mar 16;1860(6):1334–1342. doi: 10.1016/j.bbagen.2016.03.021

Figure 7. Kinetics of ergothioneine transport in CHO cells transfected with normal and chimeric OCTN cDNAs.

Figure 7

Ergothioneine (1-100 μM) transport was measured for 60 minutes at 37°C in the presence of 140 mM sodium. Non-specific ergothioneine transport, measured in the presence of 1 mM unlabeled substrate, was subtracted from each point. Data are means ± SE of triplicates (SE not shown when smaller than the size of the symbols). Black lines represent the best fit of data to a Michaelis-Menten equation. Parameters for ergothioneine transport are expressed as means ± SD. Units for Vmax are nmol/ml cell water/h. All panels have the same scale to allow direct comparison.