Fig 1. Betaine levels are increased in FLT mouse livers.

Mass spectrometry-based metabolomics was performed on n = 6 mice per group as described in Methods and Materials. Mass spectra corresponding to choline and related metabolites were identified and extracted chromatographic peaks used for relative quantification. Mann-Whitney analysis of results showed a significant increase in levels of betaine in FLT mice as compared with AEM controls, with a concomitant decrease in concentrations of metabolic precursors. *P < 0.05; data are mean ± SEM.