Fig 5. Products of PPARα-mediated thioesterase activity are upregulated in spaceflight mice.

A) Hepatic PPARα mRNA expression was quantified using qRT-PCR and normalized to 18S rRNA levels for both FLT mice and AEM controls. An unpaired t-test with Welch’s correction was used for comparison. PPARα targets include acyl-coA thioesterases, which act in different cellular compartments to mediate lipid metabolism. Mass spectrometry-based metabolomics analysis of liver compounds showed increased concentrations of products of B) ACOT2, C) ACOT1 and D) ACOT8 activity. Data (mean ± SEM) were plotted for n = 3–5 mice/group.