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. 2015 Dec 15;44(7):3082–3094. doi: 10.1093/nar/gkv1358

Figure 4.

Figure 4.

Depletion of CTCF and cohesin complex has a dramatic impact on chromatin structure at the CFTR locus. 4C interaction profiles of the CFTR locus in Caco2 cells after CTCF/Rad21 knockdown (KD). Top right inset. Efficacy of CTCF/Rad21 KD: western blots of cell lysates from Caco2 cells co-transfected with CTCF, RAD21-specific or negative control (NC) siRNAs, probed with antibodies to CTCF, RAD21 and β-tubulin. 4C-seq viewpoints are at the CFTR promoter and −20.9 kb in negative control or CTCF/Rad21 double knockdown (KD) Caco2 cells. CTCF ChIP-seq data combined from multiple cell types are from ENCODE (62).

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