Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jan 26;44(7):3204–3218. doi: 10.1093/nar/gkw036

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 1. — ΔNp63 is essential for proliferation but not survival in squamous cell carcinoma (SCC). (A) Western Blot evaluating knockdown efficiency and specificity of siRNAs targeting p63 (p63si-S1/2,-R1/2) in the head and neck SCC (HNSCC) cell line UT-SCC-74A compared to controls (mock, nsi); β-actin: loading control. (B) Colony formation assay of HNSCC cell lines after p63 knockdown. (C) Relative cell titer of p63-depleted HNSCC cell lines. Bars show mean relative cell titer +SD normalized to mock treated cells (n = 3). PLK1si: positive control. (D) SubG1 flow cytometry analysis of propidium iodide-stained cells after p63-depletion. Bars show mean % +SEM (n = 2). PLK1si: positive control. (E) Representative immunofluorescence images illustrating EdU incorporation by proliferating UT-SCC-74A after p63-depletion. DAPI: nuclear counterstain. (F) Quantification of EdU-positive cells by immunofluorescence after p63-depletion. Bars show mean % +SD of EdU-positive cells (n = 3).