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. 2016 Jan 26;44(7):3204–3218. doi: 10.1093/nar/gkw036

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — ΔNp63 regulates DNA damage repair. UT-SCC-74A were transfected with siRNAs for 48 h followed by cisplatin treatment [8 μM] for 2 h. Cells were analyzed by immunofluorescence at indicated time points. Cisplatin response is indicated as sensitive or resistant. (A and B) Detection of platinum-DNA adducts with Pt-[GG]-specific antibody (28) 6 h after cisplatin treatment. (A) Representative immunofluorescence images of Pt-[GG] adducts. DAPI: nuclear counterstain. (B) Quantification of platinum-DNA adducts from A by single-cell analysis. Box plots show fluorescence intensities [FU] of single cells, whiskers: 10th and 90th percentiles. (C–E) Time course experiment for analysis of DNA damage induction and repair. (C) Experimental setup: siRNA transfection, cisplatin treatment (for 2 h) and time points of analysis (early, 2 + 6 h and late, 2 + 46 h) are indicated. (D) Representative immunofluorescence images of DNA damage markers γH2A.X and serine 33-phosphorylated (pS33) RPA32. DAPI: nuclear counterstain. (E) Quantification of DNA damage markers from (D). Bars show mean % +SEM (n = 2) of cells positive for γH2A.X (top) and pS33-RPA32 (bottom).