Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Feb 11;44(7):3390–3407. doi: 10.1093/nar/gkw074

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 2. — The 5’ border of the CDV 5’UTR IRES. (A) Schematic representation of monocistronic (MC) and dicistronic (DC) CDV mRNAs, with the CDV 5’UTR and a stable hairpin (thick line) placed upstream of the first and/or the second cistron. (B-D) Translational activity of the IRES in (B and D) RRL and (C and D) HeLa cell-free translation extract, and its sensitivity to inhibition by dominant-negative eIF4A^R362Q. Luciferase and DC Aichivirus mRNAs (31) were used as controls for the efficiency of translation. (E) Toe-print analysis of 80S ribosomes formed on wt CDV MC mRNA in RRL in the presence of cycloheximide. (F) Model of the IRES, with arrows indicating the borders of 5’-terminal truncations of the 5’UTR. (G and H) Effect of these truncations, introduced into Stem-MC mRNA, on IRES activity in (G) RRL and (H) HeLa cell-free translation extract.