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. 2016 Feb 20;44(7):3448–3463. doi: 10.1093/nar/gkw103

Figure 7.

Figure 7.

Mapping of the interaction with histone H3 amino-terminal peptides. (A) Histograms showing the averaged backbone chemical shift perturbations (CSP) observed in 1H–15N HSQC of PHDVC5HCHNSD1 (0.2 mM) upon addition of a twelve-fold excess of H31–21, H3K4me31–21, H3K9me31–21. The last two bars in the plot correspond to the indole amides of W2157NSD1 and W2168NSD1, respectively. The dotted line represents <CSP>, the dashed line represents <CSP> + 1σ, where σ corresponds to standard deviation. Missing bars correspond to either Prolines or amides that are not visible because of exchange with the solvent. (B) Surface and cartoon representation of PHDVC5HCHNSD1. Residues showing significant chemical shift perturbation (CSP > <CSP>+1σ) are represented in cyan.