Dopamine and acetylcholine content. A) Slc10a4Δ/Δ mice had significantly reduced dopamine content measured by HPLC analysis in both the dorsal and ventral striatum as compared with Slc10a4+/+ mice (2-way ANOVA with Sidak’s post hoc comparison test, *p<0.05, **p<0.01). B) By comparison, Slc10a4Δ/Δ mice had increased DOPAC concentrations in the same regions (2-way ANOVA with Sidak’s post hoc comparison test, *p<0.05, **p<0.01). Additionally, ACh was reduced in Slc10a4Δ/Δ mice in both B) the brainstem (t-test, **p<0.01) and C) the hippocampus as compared with Slc10a4+/+ mice (t-test, *p<0.05).