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. 2016 Feb 2;9(2):119–129. doi: 10.1161/CIRCGENETICS.115.001254

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© 2016 The Authors.

Circulation: Cardiovascular Genetics is published on behalf of the American Heart Association, Inc., by Wolters Kluwer. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDervis License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 5. — Proteomic analysis of adult Drosophila hemolymph. Hemolymph was collected from adult flies and the proteome analyzed. A, Number of different proteins identified in the hemolymph of adult control (w¹¹¹⁸) and mutant dKlf15^NN flies. B, The number of proteins predicted to have a signal peptide by SignalP 4.1. C, Heat map showing a truncated list of proteins identified only in the hemolymph of dKlf15^NN mutants. Proteins are rank ordered according to spectral count; each row represents 3 independent samples from each genotype (Table I in the Data Supplement). D, Coverage of secreted protein acidic and rich in cysteine (SPARC) protein sequence by detected peptides (yellow corresponds to regions detected in proteomics).