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. Author manuscript; available in PMC: 2016 Apr 20.
Published in final edited form as: Cell Rep. 2016 Apr 7;15(3):451–459. doi: 10.1016/j.celrep.2016.03.044

Figure 2.

Figure 2

Hypoxic induction of HIF-1α protein and target gene expression is inhibited in the epidermis of TFAM EpiKO mice. (A) Representative immunohistochemical analysis of HIF-1α protein expression in the epidermis of wild-type and TFAM EpiKO mice. (B) Real Time PCR analysis of HIF-1α and TFAM mRNA expression in the epidermis of wild-type and TFAM EpiKO mice. (C) Representative Western blot analysis of HIF-1α and TFAM protein levels in epidermal lysates prepared from wild-type and TFAM EpiKO mice. Mice were exposed to normoxia (21% O2) or hypoxia (9% O2) for 16 hours. (D) Densitometric quantification of (C). HIF-1α protein levels were normalized to β-actin levels. (E) Real Time PCR analysis of PGK1, CAIX, Pai1, VEGF, and BNIP3 mRNA expression in epidermal lysates prepared from wild-type and TFAM EpiKO mice. Mice were exposed to normoxia or hypoxia for 16 hours. Charts represent means +/− SEM. N=5 mice per genotype per condition. mRNA expression was normalized to RPL19 levels. Significance was determined by one way ANOVA using Bonferonni’s post-test.