Table 1.

List of compound sequences and summary of Kd values against YopH-NT determined by 2D NMR titrations and/or ITC.

ID	Ac-Peptide sequence-NH2							Kd value (μM)
	P-1	P0	P1	P2	P3	P4	P5	YopH NT by NMR	YopH NT by ITC
1	D	E	pY	D	D	P	F	nd	0.18
2			pY	cPG	D			17.29	6.60
3	D	E	pY	cPG	D	P	F	nd	0.18
4			pY	cPG	D	I		1.47	4.40
5			pY	cPG	D	F		1.66	5.50
6			pY	cPG	D	K		62.69	14.20
7			pY	cPG	D	D		4.93	6.50
8			pY	cPG	D	P		nd	1.40
9		I	pY	cPG	D			10.47	10.90
10		F	pY	cPG	D			6.06	9.30
11		K	pY	cPG	D			127.60	nb
12		D	pY	cPG	D			21.67	16.20
13		I	pY	cPG	D	P		nd	0.52
14		F	pY	cPG	D	P		nd	0.31
15		F	pY	cPG	W	P		nd	3.10

The amino acid abbreviation pY stands for phosphorylated tyrosine, cPG stands for cyclopentyl glycine. nd indicates not determined; nb indicates no binding detected at the used experimental conditions. In each peptide, the N-terminus was acetylated and the C-terminus amidated.