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. 2016 Apr 21;7:101. doi: 10.3389/fphar.2016.00101

FIGURE 5.

FIGURE 5

Mitophagy in H/R-treated H9C2 cells. (A) Representative immunostaining of co-localization of PINK1 and COX IV. (B) Co-localization ratio of PINK1 and COX IV. (P < 0.0001, One-way ANOVA). (C) Representative immunostaining of co-localization of Parkin and COX IV. (D) Co-localization ratio of PINK1 and COX IV (P < 0.0001, One-way ANOVA). (E) Representative gel blots of expression of PINK1, Parkin and COX IV (mitochondrial loading control). Quantification analysis of (F) PINK1 expression, (G) Parkin expression (P < 0.0001, P = 0.003, One-way ANOVA). Representative images are shown (magnification, 630×). Scale bar: 20 μm. Representative immunostaining of 3 independent experiments. Data are mean ± SD from 3 independent experiments. ∗P < 0.05 vs. CON; #P < 0.05 vs. H/R.