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. 2016 Apr 21;7:101. doi: 10.3389/fphar.2016.00101

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Copyright © 2016 Ji, Wei, Hao, Xing, Yuan, Wang, Xu and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of CCCP on ALDH2 activity and expression, cell viability and 4HNE accumulation in H/R-treated H9C2 cells. (A) ALDH2 enzymatic activity (P < 0.0001, One-way ANOVA). (B) Cell viability (P < 0.0001, One-way ANOVA). (C) Representative gel blots of protein expression of ALDH2 and Actin (loading control). (D) Quantification analysis of ALDH2 expression (P = 0.5138, One-way ANOVA). (E) Representative gel blots of expression of 4HNE. (F) Quantification analysis of 4HNE expression (P = 0.001, One-way ANOVA). Data are mean ± SD from 3 independent experiments. ^∗P < 0.05 vs. H/R; ^#P < 0.05 vs. H/R + CCCP.