Figure 2.

Purification of Spa47. (A) N‐terminal chitin binding domain (CBD) and Intein tagged version of Spa47 used for overexpression and purification. The site of cleavage is shown. (B) SDS‐PAGE of Spa47 at various stages during protein purification. Whole cell lysate (WC), clarified supernatant (Sup), cleaved protein after elution from the chitin column (Chitin) and, purified Spa47 from the Q‐sepharose (Q) are shown. ** and * denote the corresponding positions of the uncleaved CBD‐Intein‐Spa47 and cleaved Spa47, respectively.