Figure 2.

Rolipram prevents H₂O₂-induced [Ca²⁺]_i signals and intracellular ROS production in SMG acinar cells. (a) Changes in [Ca²⁺]_i induced by 1 mM H₂O₂. Changes in [Ca²⁺]_i induced by 1 mM H₂O₂, pretreatment with 10 μg/mL rolipram (b) and with 10 μM 3-AB (c). The traces are averaged traces (n = 4). The upper bars indicate the extracellular solutions applied to the cells. (d) Analysis of H₂O₂-induced maximum [Ca²⁺]_i peak as determined using R340/380 fluorescence ratios (^∗ P < 0.01). (e) Expression of PARP-1 (green) in SMG tissue. (f) Effect of inflammatory mediators for 30 min in the absence or presence of rolipram (R) on intracellular ROS production in SMG cells. Results are expressed as ratio of control and mean ± SEMs are shown (n = 3, ^∗ P < 0.01 compared with control).