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. 2016 Apr 21;7:516. doi: 10.3389/fpls.2016.00516

Table 4.

Expression stability ranking of 10 candidate reference genes.

Method 1 2 3 4 5 6 7 8 9 10
RANKING ORDER IN FD (BETTER—GOOD—AVERAGE)
geNorm GAPDH/EF-1α UBC PP2A TIP41 ACTIN α-TUB β-TUB CYP SAMS
NormFinder GAPDH UBC EF-1α PP2A TIP41 ACTIN α-TUB β-TUB CYP SAMS
Bestkeeper PP2A α-TUB TIP41 GAPDH UBC ACTIN EF-1α CYP SAMS β-TUB
RefFinder GAPDH UBC EF-1α PP2A TIP41 α-TUB ACTIN β-TUB CYP SAMS
RANKING ORDER IN XS (BETTER—GOOD—AVERAGE)
geNorm UBC/GAPDH TIP41 EF-1α PP2A CYP α-TUB β-TUB ACTIN SAMS
NormFinder GAPDH TIP41 UBC EF-1α PP2A CYP ACTIN α-TUB β-TUB SAMS
Bestkeeper UBC GAPDH TIP41 PP2A EF-1α ACTIN α-TUB CYP β-TUB SAMS
RefFinder GAPDH UBC TIP41 EF-1α PP2A CYP ACTIN α-TUB β-TUB SAMS
RANKING ORDER IN QH (BETTER—GOOD—AVERAGE)
geNorm UBC/EF-1α TIP41 GAPDH PP2A ACTIN SAMS α-TUB CYP β-TUB
NormFinder TIP41 EF-1α UBC GAPDH PP2A ACTIN α-TUB CYP SAMS β-TUB
Bestkeeper CYP UBC EF-1α TIP41 α-TUB GAPDH β-TUB PP2A ACTIN SAMS
RefFinder EF-1α UBC GAPDH TIP41 CYP PP2A ACTIN α-TUB SAMS β-TUB
RANKING ORDER IN TOTAL SAMPLES (BETTER—GOOD—AVERAGE)
geNorm PP2A/TIP41 GAPDH UBC EF-1α ACTIN CYP α-TUB SAMS β-TUB
NormFinder UBC TIP41 GAPDH PP2A EF-1α ACTIN CYP α-TUB β-TUB SAMS
Bestkeeper UBC EF-1α TIP41 CYP PP2A GAPDH α-TUB ACTIN β-TUB SAMS
RefFinder TIP41 UBC GAPDH PP2A EF-1α CYP ACTIN α-TUB β-TUB SAMS

FD, ‘Feng Dan’; XS, ‘Xi Shi’; QH, ‘Que Hao.’