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. 2016 Apr 21;6:24698. doi: 10.1038/srep24698

Figure 3. Overexpressing mutants affect infection progression of F. graminearum in wheat plants.

Figure 3

Wheat plants (cv. Nandu) were infected with the wild type and overexpressing mutants carrying a constitutive EGFP reporter gene. Bright field microscopy (BF) shows browning and bleaching of the infected floret. GFP reveals colonization of the caryopsis and adjacent spikelets by the wild type, DHSoex and DHSoex/DOHHoex strains. Water was used as a negative control. The DOHHoex mutant shows minimal colonization, whereas the DHSoex shows faster colonization. (A) Micrographs of spikelet transversal sections at 3 dpi (3 d, scale bar = 2 mm) or 7 dpi (7 d, scale bar = 0.5 mm). RN: Rachis node. (B) Infection progression inside a spike at 10 dpi. Arrows mark the points of inoculation. Micrographs were taken with a fluorescence stereomicroscope (MZFLIII, Leica) and are representative of 10 infected spikes.