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. 2016 Apr 19;7:11182. doi: 10.1038/ncomms11182

Figure 1. Purification of centralspindlin components from the baculovirus expression system.

Figure 1

(a) Commassie blue-stained SDS–polyacrylamide gel (8%) shows eluates of full-length centralspindlin, Pav/kinesin-6, Tum/RacGAP and a negative control (protein purified from sf9 cells infected with no-gene-bearing baculoviruses) obtained from Ni-NTA affinity column. (b) Commassie blue-stained SDS–polyacrylamide gel (20%) shows fragment Tum1–65 eluate obtained from Ni-NTA affinity column. (c) Commassie blue-stained SDS–polyacrylamide gel shows the composition of protein fractions obtained during the purification of centralspindlin from the gel-filtration chromatography (Superose 6 FPLC, GE Pharmacia). (d) Corresponding plot of protein concentration versus fraction number demonstrates the centralspindlin complex elutes in a single peak.