Figure 2. Purified centralspindlin and Pav/kinesin-6 both bundle MTs.
Fluorescence microscopy demonstrates that purified centralspindlin (a) and Pav/kinesin-6 (b) have obvious bundling activity, while Tum/RacGAP (c) or kinesin-1 head dimer (d) cannot bundle MTs under the same conditions (1 mM ATP and 150 mM KCl). Scale bar, 10 μm.