Table 1. Hydrodynamic properties of various centralspindlin components.
S value (1 × 10−13 s) | Rs (nm) | MWholo (kD) | MWsub (kD) | Subunit# | |
---|---|---|---|---|---|
Pav/kinesin-6 alone | 5.72 | 8.95 | 214 | 107 | 2 |
Tum/RacGAP alone | 4.44 | 6.22 | 116 | 65 | 2 |
Centralspindlin from coexpression | 8.70 | 10.23 | 372 | 107 (Pav)65 (Tum) | 4 (2 Pav+2 Tum) |
Centralspindlin from in vitro add-back | 8.65 | 9.74 | 352 | 107 (Pav)65 (Tum) | 4 (2 Pav+2 Tum) |
MW, molecular weight.